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shRNA necklace V | silver pp_lab anyone for whom calculus wasshRNA necklace V sterling silver If you have ever designed an shRNA construct, picked a hairpin sequence from a library, or run a knockdown screen and waited two days for the western blot, you already know what this is. The hairpin RNA that lets a single sequence silence a chosen gene with reasonable specificity. The Science of shRNA Short hairpin RNAs combine a sense strand, a loop, and an antisense strand designed to base pair into the hairpin